Review



primescripttm one step rt pcr kit  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 98
      Buy from Supplier

    Structured Review

    TaKaRa primescripttm one step rt pcr kit
    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template <t>for</t> <t>RT-PCR</t> to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.
    Primescripttm One Step Rt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 10975 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+one+step+rt+pcr+kit/PrimeScript+RT-PCR+Kit/pmc13195868-92-16-21
    Average 98 stars, based on 10975 article reviews
    primescripttm one step rt pcr kit - by Bioz Stars, 2026-10
    98/100 stars

    Images

    1) Product Images from "Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves"

    Article Title: Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves

    Journal: Veterinary Research

    doi: 10.1186/s13567-026-01765-3

    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template for RT-PCR to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.
    Figure Legend Snippet: Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template for RT-PCR to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.

    Techniques Used: Isolation, Reverse Transcription Polymerase Chain Reaction, Sequencing, Amplification, Marker, Generated, Clone Assay, Construct, Recombinant, Negative Control, Control

    Related Articles

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Inhibitory effect of cannabidiol on infectious bronchitis virus in chickens in vitro and in vivo.
    Article Snippet: Infectious bronchitis (IB) is a highly contagious and economically significant disease of chickens that adversely impacts global poultry production.. The causative agent, infectious bronchitis virus (IBV), belongs to the genus Gammacoronavirus within the family Coronaviridae.. IBV infection primarily causes respiratory distress, nephritis with urate deposition, secondary bacterial infections, and mortality in affected flocks (Cavanagh 2007).

    Article Title: Discovery and molecular identification of a new orthophasmavirus in the Asian citrus psyllid ( Diaphorina citri )
    Article Snippet: .. RT-PCR was conducted using a PrimeScriptTM One Step RT-PCR Kit (Takara, Dalian, China) in a 10 μL reaction system consisting of 1 μL RNA template, 0.4 μL Enzyme Mix, 0.4 μL of each specific primer , and 1.8 μL ddH2O. ..

    Article Title: Uncomplexed-TSC1 deploys novel mTORC1-independent pathway to exacerbate the liver glycogen storage in TSC.
    Article Snippet: Finally, the bands were visualized using the enhanced chemiluminescence kit (Millipore, Massachusetts). qRT-PCR Total RNA was extracted using TRIzol method, and cDNA was synthesized using PrimeScriptTM RT reagent Kit with gDNA Eraser (Takara, RR047A) according to the manufacturer’s instructions. .. The Takara PrimeScriptTM One Step RT-PCR Kit was employed for the RT-PCR assay (Takara, RR055A). ..

    Article Title: Uncomplexed-TSC1 deploys novel mTORC1-independent pathway to exacerbate the liver glycogen storage in TSC
    Article Snippet: Total RNA was extracted using TRIzol method, and cDNA was synthesized using PrimeScriptTM RT reagent Kit with gDNA Eraser (Takara, RR047A) according to the manufacturer’s instructions. .. The Takara PrimeScriptTM One Step RT-PCR Kit was employed for the RT-PCR assay (Takara, RR055A). ..

    One Step RT-PCR:

    Article Title: Inhibitory effect of cannabidiol on infectious bronchitis virus in chickens in vitro and in vivo.
    Article Snippet: Infectious bronchitis (IB) is a highly contagious and economically significant disease of chickens that adversely impacts global poultry production.. The causative agent, infectious bronchitis virus (IBV), belongs to the genus Gammacoronavirus within the family Coronaviridae.. IBV infection primarily causes respiratory distress, nephritis with urate deposition, secondary bacterial infections, and mortality in affected flocks (Cavanagh 2007).

    Article Title: Discovery and molecular identification of a new orthophasmavirus in the Asian citrus psyllid ( Diaphorina citri )
    Article Snippet: .. RT-PCR was conducted using a PrimeScriptTM One Step RT-PCR Kit (Takara, Dalian, China) in a 10 μL reaction system consisting of 1 μL RNA template, 0.4 μL Enzyme Mix, 0.4 μL of each specific primer , and 1.8 μL ddH2O. ..

    Article Title: Unveiling the RNA viral diversity in three organs of the Asian house shrew ( Suncus murinus ) from Tropical Hainan, China: a previously underappreciated key zoonotic reservoir
    Article Snippet: .. Amplifications were conducted using the PrimeScriptTM One Step RT–PCR kit (Takara) in 25 μL reaction volumes. .. The thermal cycling protocol consisted of 50 °C for 30 min (reverse transcription); 94 °C for 2 min (initial denaturation); 35 cycles of 94 °C for 30 s, primer-specific annealing for 30 s, and 72 °C for 1 min; and a final extension at 72 °C for 10 min. All the PCR products (with sizes ranging from 537–1,200 bp) were subsequently purified and subjected to Sanger sequencing by Tianyi Huiyuan Biotechnology.

    Article Title: Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves
    Article Snippet: Total RNA was extracted from PBMCs using TRIzol reagent (Ambion, Austin, TX, USA) according to the manufacturer’s instructions. .. The extracted RNA was directly subjected to one-step reverse-transcription polymerase chain reaction (RT-PCR) amplification using the PrimeScriptTM One Step RT-PCR Kit (TaKaRa, Shiga, Japan), which allows reverse transcription and PCR amplification to be conducted in a single reaction. ..

    Article Title: Effective strategies for pathogen reduction in decentralized wastewater treatment systems.
    Article Snippet: Viral RNA was detected by RT-qPCR on a Mx3000P qPCR system (Stratagene; USA) instrument. .. PrimeScriptTM One Step RT-PCR Kit (Takara Bio, USA) was used in a 10 μl total volume, using 2.5 μl of extracted RNA. ..

    Article Title: Uncomplexed-TSC1 deploys novel mTORC1-independent pathway to exacerbate the liver glycogen storage in TSC.
    Article Snippet: Finally, the bands were visualized using the enhanced chemiluminescence kit (Millipore, Massachusetts). qRT-PCR Total RNA was extracted using TRIzol method, and cDNA was synthesized using PrimeScriptTM RT reagent Kit with gDNA Eraser (Takara, RR047A) according to the manufacturer’s instructions. .. The Takara PrimeScriptTM One Step RT-PCR Kit was employed for the RT-PCR assay (Takara, RR055A). ..

    Article Title: Uncomplexed-TSC1 deploys novel mTORC1-independent pathway to exacerbate the liver glycogen storage in TSC
    Article Snippet: Total RNA was extracted using TRIzol method, and cDNA was synthesized using PrimeScriptTM RT reagent Kit with gDNA Eraser (Takara, RR047A) according to the manufacturer’s instructions. .. The Takara PrimeScriptTM One Step RT-PCR Kit was employed for the RT-PCR assay (Takara, RR055A). ..

    Article Title: Lycium barbarum polysaccharide LBP-4 attenuates triptolide-induced male reproductive toxicity via Nrf2/Keap1-mediated antioxidant response and apoptotic regulation.
    Article Snippet: Ethnopharmacological relevance: Lycium barbarum (Goji berry) has been traditionally employed to enhance vitality and treat reproductive deficiencies.. Polysaccharides derived from its fruit (LBP) are key bioactive constituents, historically linked to nourishing the male reproductive system and mitigating oxidative stress—a practice now supported by modern research on their protective mechanisms.. Ethnopharmacological relevance: Lycium barbarum, commonly known as Goji berry, has been traditionally utilized in herbal medicine to enhance vitality and address reproductive health disorders.

    Reverse Transcription:

    Article Title: Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves
    Article Snippet: Total RNA was extracted from PBMCs using TRIzol reagent (Ambion, Austin, TX, USA) according to the manufacturer’s instructions. .. The extracted RNA was directly subjected to one-step reverse-transcription polymerase chain reaction (RT-PCR) amplification using the PrimeScriptTM One Step RT-PCR Kit (TaKaRa, Shiga, Japan), which allows reverse transcription and PCR amplification to be conducted in a single reaction. ..

    Article Title: Lycium barbarum polysaccharide LBP-4 attenuates triptolide-induced male reproductive toxicity via Nrf2/Keap1-mediated antioxidant response and apoptotic regulation.
    Article Snippet: Ethnopharmacological relevance: Lycium barbarum (Goji berry) has been traditionally employed to enhance vitality and treat reproductive deficiencies.. Polysaccharides derived from its fruit (LBP) are key bioactive constituents, historically linked to nourishing the male reproductive system and mitigating oxidative stress—a practice now supported by modern research on their protective mechanisms.. Ethnopharmacological relevance: Lycium barbarum, commonly known as Goji berry, has been traditionally utilized in herbal medicine to enhance vitality and address reproductive health disorders.

    Polymerase Chain Reaction:

    Article Title: Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves
    Article Snippet: Total RNA was extracted from PBMCs using TRIzol reagent (Ambion, Austin, TX, USA) according to the manufacturer’s instructions. .. The extracted RNA was directly subjected to one-step reverse-transcription polymerase chain reaction (RT-PCR) amplification using the PrimeScriptTM One Step RT-PCR Kit (TaKaRa, Shiga, Japan), which allows reverse transcription and PCR amplification to be conducted in a single reaction. ..

    Amplification:

    Article Title: Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves
    Article Snippet: Total RNA was extracted from PBMCs using TRIzol reagent (Ambion, Austin, TX, USA) according to the manufacturer’s instructions. .. The extracted RNA was directly subjected to one-step reverse-transcription polymerase chain reaction (RT-PCR) amplification using the PrimeScriptTM One Step RT-PCR Kit (TaKaRa, Shiga, Japan), which allows reverse transcription and PCR amplification to be conducted in a single reaction. ..



    Similar Products

    primescripttm one step rt pcr kit  (TaKaRa)
    98
    TaKaRa primescripttm one step rt pcr kit
    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template <t>for</t> <t>RT-PCR</t> to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.
    Primescripttm One Step Rt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+one+step+rt+pcr+kit/PrimeScript+RT-PCR+Kit/pmc13195868-92-16-21
    Average 98 stars, based on 1 article reviews
    primescripttm one step rt pcr kit - by Bioz Stars, 2026-10
    98/100 stars
    		  Buy from Supplier
    one step sybr primescripttm rt pcr kit  (TaKaRa)
    97
    TaKaRa one step sybr primescripttm rt pcr kit
    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template <t>for</t> <t>RT-PCR</t> to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.
    One Step Sybr Primescripttm Rt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+one+step+rt+pcr+kit/One+Step+PrimeScript+RT-PCR+Kit/pm42134762-109-6-14
    Average 97 stars, based on 1 article reviews
    one step sybr primescripttm rt pcr kit - by Bioz Stars, 2026-10
    97/100 stars
    		  Buy from Supplier
    one step primescripttm rt pcr kit  (TaKaRa)
    97
    TaKaRa one step primescripttm rt pcr kit
    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template <t>for</t> <t>RT-PCR</t> to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.
    One Step Primescripttm Rt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+one+step+rt+pcr+kit/One+Step+PrimeScript+RT-PCR+Kit/pmc13128697-57-7-15
    Average 97 stars, based on 1 article reviews
    one step primescripttm rt pcr kit - by Bioz Stars, 2026-10
    97/100 stars
    		  Buy from Supplier
    primescripttm ii high fidelity one step rt pcr kit  (TaKaRa)
    95
    TaKaRa primescripttm ii high fidelity one step rt pcr kit
    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template <t>for</t> <t>RT-PCR</t> to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.
    Primescripttm Ii High Fidelity One Step Rt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+one+step+rt+pcr+kit/High+Fidelity+one-step+RT-PCR/pm42056564-62-12-19
    Average 95 stars, based on 1 article reviews
    primescripttm ii high fidelity one step rt pcr kit - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    primescripttm rt qpcr kit  (TaKaRa)
    96
    TaKaRa primescripttm rt qpcr kit
    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template <t>for</t> <t>RT-PCR</t> to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.
    Primescripttm Rt Qpcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+one+step+rt+pcr+kit/One-Step+TB+Green+PrimeScript+RT-PCR+Kit+II/pmc13104215-77-7-11
    Average 96 stars, based on 1 article reviews
    primescripttm rt qpcr kit - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    one step tb green primescripttm rt pcr kit ii  (TaKaRa)
    96
    TaKaRa one step tb green primescripttm rt pcr kit ii
    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template <t>for</t> <t>RT-PCR</t> to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.
    One Step Tb Green Primescripttm Rt Pcr Kit Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+one+step+rt+pcr+kit/One-Step+TB+Green+PrimeScript+RT-PCR+Kit+II/pmc13119100-259-33-43
    Average 96 stars, based on 1 article reviews
    one step tb green primescripttm rt pcr kit ii - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template for RT-PCR to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.

    Journal: Veterinary Research

    Article Title: Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves

    doi: 10.1186/s13567-026-01765-3

    Figure Lengend Snippet: Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template for RT-PCR to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.

    Article Snippet: The extracted RNA was directly subjected to one-step reverse-transcription polymerase chain reaction (RT-PCR) amplification using the PrimeScriptTM One Step RT-PCR Kit (TaKaRa, Shiga, Japan), which allows reverse transcription and PCR amplification to be conducted in a single reaction.

    Techniques: Isolation, Reverse Transcription Polymerase Chain Reaction, Sequencing, Amplification, Marker, Generated, Clone Assay, Construct, Recombinant, Negative Control, Control